Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- ARG54703 - Provider product page
- Provider
- Arigo
- Product name
- anti-EZH2 antibody
- Antibody type
- Polyclonal
- Antigen
- Human EZH2 recombinant protein (NP_001190176.1).
- Description
- Protein A purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue lysates (from left to right) stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of lysates from Jurkat, T47D cell line (from left to right) stained with ARG54703 anti-EZH2 antibody at 1:2000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: 293 cell stained with ARG54703 anti-EZH2 antibody. Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: human testis tissue stained with ARG54703 anti-EZH2 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: Jurkat cells stained with ARG54703 anti-EZH2 antibody (bottom histogram) or without primary antibody control (top histogram).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG54703 anti-EZH2 antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.