M00050-1

antibody from Boster Biological Technology

Targeting: EZH2 ENX-1, EZH1, KMT6, KMT6A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00050-1

Provider

Boster Biological Technology

Product name

Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for KMT6/EZH2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: FCM, IF, IHC, ICC, WB. Reactive species: Human KMT6/EZH2 information: Molecular Weight: 85363 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.

Reactivity

Human

Host

Rabbit

Antibody clone number

IDF-5

Vial size

100ug/vial

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of KMT6 / EZH2 expression in HEK293 cell lysate (M00050-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH2 monoclonal antibody (Catalog # M00050-1) overnight at 4

Additional image