PAB0648

antibody from Abnova Corporation

Targeting: EZH2 ENX-1, EZH1, KMT6, KMT6A

Western blot (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0648

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0648, RRID:AB_1017095

Product name

Ezh2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against partial recombinantt Ezh2.

Storage

Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The primary microRNA-208b interacts with Polycomb-group protein, Ezh2, to regulate gene expression in the heart.

Mathiyalagan P, Okabe J, Chang L, Su Y, Du XJ, El-Osta A
Nucleic acids research 2014 Jan;42(2):790-803

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Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo.

Rugg-Gunn PJ, Cox BJ, Ralston A, Rossant J
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 15;107(24):10783-90

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Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation.

Kuzmichev A, Margueron R, Vaquero A, Preissner TS, Scher M, Kirmizis A, Ouyang X, Brockdorff N, Abate-Shen C, Farnham P, Reinberg D
Proceedings of the National Academy of Sciences of the United States of America 2005 Feb 8;102(6):1859-64

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EZH2 is a marker of aggressive breast cancer and promotes neoplastic transformation of breast epithelial cells.

Kleer CG, Cao Q, Varambally S, Shen R, Ota I, Tomlins SA, Ghosh D, Sewalt RG, Otte AP, Hayes DF, Sabel MS, Livant D, Weiss SJ, Rubin MA, Chinnaiyan AM
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 30;100(20):11606-11

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The polycomb group protein EZH2 is involved in progression of prostate cancer.

Varambally S, Dhanasekaran SM, Zhou M, Barrette TR, Kumar-Sinha C, Sanda MG, Ghosh D, Pienta KJ, Sewalt RG, Otte AP, Rubin MA, Chinnaiyan AM
Nature 2002 Oct 10;419(6907):624-9

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Transcriptional repression mediated by the human polycomb-group protein EED involves histone deacetylation.

van der Vlag J, Otte AP
Nature genetics 1999 Dec;23(4):474-8

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis using the Ezh2 polyclonal antibody (Cat # PAB0648).Extract of insect cells expressing recombinant Flag-Ezh2 (BacFlag-Ezh2) (lane 1) and increasing amounts of Jurkat nuclear extract (lanes 2-4) were analysed by Western blot using the antibody directed against Ezh2 at dilution 1 : 2000. Size and location of the protein are indicated.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis using the Ezh2 polyclonal antibody (Cat # PAB0648). In lane 2, HeLa nuclear extract (HeLa NE) was analysed by Western blot using the Ezh2 polyclonal antibody (Cat # PAB0648) at a dilution of 1 : 1000 in TBS-Tween + 5% skimmed milk. In lane 1, a molecular weight marker is shown.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ChIP results obtained with the Ezh2 polyclonal antibody (Cat # PAB0648). ChIP assays were performed using Jurkat cells.

Validation comment

ChIP