Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Chromatin Immunoprecipitation [2]
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Validation data
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- Product number
- 17-662 - Provider product page
- Provider
- EMD Millipore
- Proper citation
- Millipore Cat#17-662, RRID:AB_11211831
- Product name
- ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set
- Antibody type
- Monoclonal
- Antigen
- EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 25 assays
- Storage
- Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Submitted references The ablation of EZH2 uncovers its crucial role in rhabdomyosarcoma formation.
Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer.
Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans.
Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2.
[Addicted patients: do not distribute street drugs: do treat with registered psychoactive medication].
Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27.
Marchesi I, Fiorentino FP, Rizzolio F, Giordano A, Bagella L
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3828-36
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3828-36
Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer.
Ren G, Baritaki S, Marathe H, Feng J, Park S, Beach S, Bazeley PS, Beshir AB, Fenteany G, Mehra R, Daignault S, Al-Mulla F, Keller E, Bonavida B, de la Serna I, Yeung KC
Cancer research 2012 Jun 15;72(12):3091-104
Cancer research 2012 Jun 15;72(12):3091-104
Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans.
Yang F, Zhang L, Huo XS, Yuan JH, Xu D, Yuan SX, Zhu N, Zhou WP, Yang GS, Wang YZ, Shang JL, Gao CF, Zhang FR, Wang F, Sun SH
Hepatology (Baltimore, Md.) 2011 Nov;54(5):1679-89
Hepatology (Baltimore, Md.) 2011 Nov;54(5):1679-89
Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2.
Pasini D, Hansen KH, Christensen J, Agger K, Cloos PA, Helin K
Genes & development 2008 May 15;22(10):1345-55
Genes & development 2008 May 15;22(10):1345-55
[Addicted patients: do not distribute street drugs: do treat with registered psychoactive medication].
De Jong CA
Nederlands tijdschrift voor geneeskunde 2007 Sep 8;151(36):1970-2
Nederlands tijdschrift voor geneeskunde 2007 Sep 8;151(36):1970-2
Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27.
Kirmizis A, Bartley SM, Kuzmichev A, Margueron R, Reinberg D, Green R, Farnham PJ
Genes & development 2004 Jul 1;18(13):1592-605
Genes & development 2004 Jul 1;18(13):1592-605
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Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG, (Part No. 12-371B), or Anti-EZH2, clone AC22 antibody and the Magna ChIPª G Kit (Cat. # 17-611). Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using Control Primers (Figure 1). Please refer to the EZ-Magna ChIPª G (Cat. # 17-409) or EZ-ChIPª (Cat. # 17-371) protocol for experimental details.
- Validation comment
- ChIP
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal mouse IgG, (Part No. 12-371B), or Anti-EZH2, clone AC22 antibody and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and MYT-1 promoter (positive) primers (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna G ChIPª (Cat. # 17-409) or EZ-ChIPª (Cat. # 17-371) protocol for experimental details.
- Validation comment
- ChIP