MA5-31745

antibody from Invitrogen Antibodies

Targeting: GRIK4 GluK4, GRIK, KA1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31745

Provider

Invitrogen Antibodies

Product name

GRIK4 Monoclonal Antibody (8H5G5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-31745 has been tested in indirect ELISA.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

8H5G5

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GRIK4 in human GRIK4 recombinant protein. Sample was incubated with GRIK4 monoclonal antibody (Product # MA5-31745) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GRIK4 in HEK293 (1) and GRIK4 cell lysate. Samples were incubated with GRIK4 monoclonal antibody (Product # MA5-31745) using a dilution of 1:500-1:2000.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of GRIK4 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with GRIK4 monoclonal antibody (Product # MA5-31745) using a dilution of 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GRIK4 in Hela cells (green). Sample was incubated with GRIK4 monoclonal antibody (Product # MA5-31745) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GRIK4 in SK-N-SH cells (green). Sample was incubated with GRIK4 monoclonal antibody (Product # MA5-31745) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GRIK4 in paraffin-embedded cervical cancer tissue. Sample was incubated with GRIK4 monoclonal antibody (Product # MA5-31745) using a dilution of 1:200-1:1000 followed by DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GRIK4 in paraffin-embedded bladder cancer tissue. Sample was incubated with GRIK4 monoclonal antibody (Product # MA5-31745) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of GRIK4 in SH-SY5Y cells (green). Sample was incubated with GRIK4 monoclonal antibody (Product # MA5-31745) using a dilution of 1:200-1:400 followed by negative control (red).