Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF1694 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Follistatin-like 1/FSTL1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse FSTL1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q12841
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Follistatin like-1 aggravates silica-induced mouse lung injury.
Follistatin-like protein 1 enhances NLRP3 inflammasome-mediated IL-1β secretion from monocytes and macrophages.
Tumor suppressor effect of follistatin-like 1 in ovarian and endometrial carcinogenesis: a differential expression and functional analysis.
Fang Y, Zhang S, Li X, Jiang F, Ye Q, Ning W
Scientific reports 2017 Mar 24;7(1):399
Scientific reports 2017 Mar 24;7(1):399
Follistatin-like protein 1 enhances NLRP3 inflammasome-mediated IL-1β secretion from monocytes and macrophages.
Chaly Y, Fu Y, Marinov A, Hostager B, Yan W, Campfield B, Kellum JA, Bushnell D, Wang Y, Vockley J, Hirsch R
European journal of immunology 2014 May;44(5):1467-79
European journal of immunology 2014 May;44(5):1467-79
Tumor suppressor effect of follistatin-like 1 in ovarian and endometrial carcinogenesis: a differential expression and functional analysis.
Chan QK, Ngan HY, Ip PP, Liu VW, Xue WC, Cheung AN
Carcinogenesis 2009 Jan;30(1):114-21
Carcinogenesis 2009 Jan;30(1):114-21
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Follistatin-like 1/FSTL1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Follistatin-like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Follistatin-like 1/FSTL1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Follistatin-like 1/FSTL1 in HeLa Human Cell Line. Follistatin-like 1/FSTL1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Follistatin-like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.