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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF6696 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat UBASH3B/STS1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and rat UBASH3B/STS1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q8TF42- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat UBASH3B/STS1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U-118-MG human glioblastoma/astrocytoma cell line, U2OS human osteosarcoma cell line, and Y3-Ag rat myeloid cell line. PVDF Membrane was probed with 2 µg/mL of Human/Rat UBASH3B/STS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6696) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBASH3B/STS1 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- UBASH3B/STS1 in HeLa Human Cell Line. UBASH3B/STS1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat UBASH3B/STS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6696) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
