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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 700242 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MNK1 (Thr197, Thr202) Recombinant Rabbit Monoclonal Antibody (18H4L11)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, chicken, chimpanzee, equine, mouse, primate, rat, Rhesus monkey , porcine, Xenopus and zebrafish based on sequence homology.
- Antibody clone number
- 18H4L11
- Concentration
- 0.5 mg/mL
Submitted references Enteral delivery of proteins stimulates protein synthesis in human duodenal mucosa in the fed state through a mammalian target of rapamycin-independent pathway.
Coëffier M, Claeyssens S, Bôle-Feysot C, Guérin C, Maurer B, Lecleire S, Lavoinne A, Donnadieu N, Cailleux AF, Déchelotte P
The American journal of clinical nutrition 2013 Feb;97(2):286-94
The American journal of clinical nutrition 2013 Feb;97(2):286-94
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-MNK pThr197/202 in HeLa cells using a Phospho-MNK pThr197/202 recombinant rabbit monoclonal antibody (Product # 700242) at a dilution of 5 µg/mL in the absence of peptide (top left) and presence of phosphopeptide used as immunogen (top right) or non-phosphopeptide (bottom left), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, center) and composite image with Phalloidin (right).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-MNK pThr197/202 in HeLa cells using a Phospho-MNK pThr197/202 recombinant rabbit monoclonal antibody (Product # 700242) at a dilution of 5 µg/mL in the absence of peptide (top left) and presence of phosphopeptide used as immunogen (top right) or non-phosphopeptide (bottom left), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, center) and composite image with Phalloidin (right).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-MNK pThr197/202 in HeLa cells using a Phospho-MNK pThr197/202 recombinant rabbit monoclonal antibody (Product # 700242) at a dilution of 5 µg/mL in the absence of peptide (top left) and presence of phosphopeptide used as immunogen (top right) or non-phosphopeptide (bottom left), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, center) and composite image with Phalloidin (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-MNK pThr197/202 in formalin-fixed, paraffin-embedded human thyroid carcinoma (top left), normal thyroid (top right) and prostate carcinoma (bottom) using a Phospho-MNK pThr197/202 monoclonal antibody (Product # 700242) at a dilution of 2 µg/mL. Staining was visualized using DAB and images were taken at a magnification of 20x. Results show nuclear and cytoplasmic staining in tumor cells and no staining in normal tissue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-MNK pThr197/202 in formalin-fixed, paraffin-embedded human thyroid carcinoma (top left), normal thyroid (top right) and prostate carcinoma (bottom) using a Phospho-MNK pThr197/202 monoclonal antibody (Product # 700242) at a dilution of 2 µg/mL. Staining was visualized using DAB and images were taken at a magnification of 20x. Results show nuclear and cytoplasmic staining in tumor cells and no staining in normal tissue.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Phospho-MNK pThr197/202 in Jurkat cells using a Phospho-MNK pThr197/202 recombinant rabbit monoclonal antibody (Product # 700242) at a dilution of 0.5ug. Cells were fixed and permeabilized using FIX & PERM (Product # GAS004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (black) compared to a control without primary antibody (gray). Pre-incubation with the phosphopeptide decreased the signal (red) whereas incubation with the non-phosphopeptide did not (blue).
