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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 13527-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13527-1-AP, RRID:AB_2096200
- Product name
- EIF5B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated EIF5B antibody (Cat. #13527-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B.
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
Kim E, Kim JH, Seo K, Hong KY, An SWA, Kwon J, Lee SV, Jang SK
Cellular and molecular life sciences : CMLS 2018 Dec;75(23):4287-4300
Cellular and molecular life sciences : CMLS 2018 Dec;75(23):4287-4300
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
Jiang X, Jiang X, Feng Y, Xu R, Wang Q, Deng H
PloS one 2016;11(12):e0168387
PloS one 2016;11(12):e0168387
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP(EIF5B antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13527-1-AP(EIF5B antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13527-1-AP(EIF5B antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
