H00009669-B01P

antibody from Abnova Corporation

Targeting: EIF5B DKFZp434I036, FLJ10524, IF2, KIAA0741

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009669-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009669-B01P, RRID:AB_1573399

Product name

EIF5B purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human EIF5B protein.

Antigen sequence

MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQ
EPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQ
GIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKK
QSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDD
DDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIK
ERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGP
NIESGNEDDDASFKIKTVAQKKAEKKERERKKRDE
EKAKLRKLKEKEELETGKKDQSKQKESQRKFEEET
VKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKK
KDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAK
LKEEEERQKREEEERIKRLEELEAKRKEEERLEQE
KRERKKQKEKERKERLKKEGKLLTKSQREARARAE
ATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIP
QQLESKEVSESMELCAAVEVMEQGVPEKEETPPPV
EPEEEEDTEDAGLDDWEAMASDEETEKVEGNTVHI
EVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGS
EGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDS
DDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLR
APIICVLGHVDTGKTKILDKLRHTHVQDGEAGGIT
QQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLI
IDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEP
QTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDS
DVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNA
ALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVE
LTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDV
ILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPM
KELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGL
PLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKG
VYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHK
KDVMKASVMLEHDPQYAVNLAFDVRIERDAQEMAD
SLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFK
HIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVK
QGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQ
EVCVKIEPIPGESPKMFGRHFEATDILVSKISRQS
IDALKDWFRDEMQKSDWQLIVELKKVFEII


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EIF5B expression in transfected 293T cell line (H00009669-T01) by EIF5B MaxPab polyclonal antibody.Lane 1: EIF5B transfected lysate(134.2 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

EIF5B MaxPab polyclonal antibody. Western Blot analysis of EIF5B expression in human placenta.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

EIF5B MaxPab polyclonal antibody. Western Blot analysis of EIF5B expression in Jurkat.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to EIF5B on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol