13527-1-AP

antibody from Invitrogen Antibodies

Targeting: EIF5B DKFZp434I036, FLJ10524, IF2, KIAA0741

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

13527-1-AP

Provider

Invitrogen Antibodies

Product name

EIF5B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TTIDVILIN GRLKEGDTII VPGVEGPIVT QIRGLLLPPP MKELRVKNQY EKHKEVEAAQ GVKILGKDLE KTLAGLPLLV AYKEDEIPVL KDELIHELKQ TLNAIKLEEK GVYVQASTLG SLEALLEFLK TSEVPYAGIN IGPVHKKDVM KASVMLEHDP QYAVNLAFDV RIERDAQEMA DSLGVRIFSA EIIYHLFDAF TKYRQDYKKQ KQEEFKHIAV FPCKIKILPQ YIFNSRDPIV MGVTVEAGQV KQGTPMCVPS KNFVDIGIVT SIEINHKQVD VAKKGQEVCV KIEPIPGESP KMFGRHFEAT DILVSKISRQ SIDALKDWFR DEMQKSDWQL IVELKKVFEI I (871-1220 aa encoded by BC032639)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 40x lens).