13527-1-AP
antibody from Invitrogen Antibodies
Targeting: EIF5B
DKFZp434I036, FLJ10524, IF2, KIAA0741
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 13527-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EIF5B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TTIDVILIN GRLKEGDTII VPGVEGPIVT QIRGLLLPPP MKELRVKNQY EKHKEVEAAQ GVKILGKDLE KTLAGLPLLV AYKEDEIPVL KDELIHELKQ TLNAIKLEEK GVYVQASTLG SLEALLEFLK TSEVPYAGIN IGPVHKKDVM KASVMLEHDP QYAVNLAFDV RIERDAQEMA DSLGVRIFSA EIIYHLFDAF TKYRQDYKKQ KQEEFKHIAV FPCKIKILPQ YIFNSRDPIV MGVTVEAGQV KQGTPMCVPS KNFVDIGIVT SIEINHKQVD VAKKGQEVCV KIEPIPGESP KMFGRHFEAT DILVSKISRQ SIDALKDWFR DEMQKSDWQL IVELKKVFEI I (871-1220 aa encoded by BC032639)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 40x lens).