PA5-69577

antibody from Invitrogen Antibodies

Targeting: POLQ POLH

Western blot (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-69577

Provider

Invitrogen Antibodies

Product name

POLQ Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This target displays homology in the following species: Cow: 93%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 86%; Rat: 100%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Molecular basis of microhomology-mediated end-joining by purified full-length Polθ.

Black SJ, Ozdemir AY, Kashkina E, Kent T, Rusanov T, Ristic D, Shin Y, Suma A, Hoang T, Chandramouly G, Siddique LA, Borisonnik N, Sullivan-Reed K, Mallon JS, Skorski T, Carnevale V, Murakami KS, Wyman C, Pomerantz RT
Nature communications 2019 Sep 27;10(1):4423

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Poltheta promotes efficient MMEJ of long ssDNA. a Schematic of Poltheta. b Schematic of MMEJ of DNA with long overhangs. c Schematic of Poltheta-pol ssDNAx via snap-back replication. d Schematic of Poltheta MMEJ of long ssDNA. e SDS gel (left) and Western blot (right) of purified human Poltheta. f Denaturing gel showing primer extension by Poltheta and Poltheta-pol. g TLC plate showing ATP hydrolysis by Poltheta (left). Plot of ATP hydrolysis in the presence of ssDNA. n = 3 +- s.d. (right). Initial rate of ATP hydrolysis = 738 pmol min -1 . h Schematic of MMEJ of long ssDNA. i , j Non-denaturing gels showing MMEJ of the indicated long ssDNA by Poltheta and Poltheta-pol at 3 nM. k , l Non-denaturing gels showing MMEJ by 3 nM of Poltheta of the indicated ssDNA (lane 2) and subsequent cleavage by XmaI (lane 3). m Bar plots showing % of MMEJ and ssDNAx products generated by 3 nM Poltheta and 3 nM Poltheta-pol on the indicated ssDNA. n = 3 (left), n = 4 (right) +- s.d. n Non-denaturing gel showing time course of MMEJ by 3 nM of Poltheta on the indicated ssDNA (left). Plot of Poltheta MMEJ time course. n = 3 +- s.d. (right). Initial rate of MMEJ = 13.5 fmol MMEJ products min -1 . o , q Non-denaturing gels showing MMEJ by indicated amounts of Poltheta (o) and Poltheta-pol (q) on indicated concentrations of 100 nt ssDNA with 6 bp microhomology. p Non-denaturing gels of MMEJ reactions performed by 3 nM of the indicated proteins on short 26