Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-16890 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- gamma Actin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Suggested positive control: Hela whole cell extract.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Single molecule poly(A) tail-seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails.
La Deletion from Mouse Brain Alters Pre-tRNA Metabolism and Accumulation of Pre-5.8S rRNA, with Neuron Death and Reactive Astrocytosis.
Mattijssen S, Iben JR, Li T, Coon SL, Maraia RJ
eLife 2020 Aug 3;9
eLife 2020 Aug 3;9
La Deletion from Mouse Brain Alters Pre-tRNA Metabolism and Accumulation of Pre-5.8S rRNA, with Neuron Death and Reactive Astrocytosis.
Blewett NH, Iben JR, Gaidamakov S, Maraia RJ
Molecular and cellular biology 2017 May 15;37(10)
Molecular and cellular biology 2017 May 15;37(10)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), COS-7 (Lane 3), MDCK (Lane 4), NIH/3T3 (Lane 5), HT-29 (Lane 6), PC-12 (Lane 7), Neuro-2a (Lane 8) and tissue extract of Mouse Brain (Lane 9). The blot was probed with gamma Actin Polyclonal Antibody (Product # PA1-16890, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to gamma Actin was observed across the cell lines and tissue tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of gamma Actin in 15 µg whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Samples were incubated in gamma Actin polyclonal antibody (Product # PA1-16890 using a dilution of 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of gamma Actin in 20 µg 3T3 lysates. Samples were incubated in gamma Actin polyclonal antibody (Product # PA1-16890). ECL detection 30 seconds. Lane 1: 1:5,000 dilution; Lane 2 - 1:10,000 dilution; Lane 3 - 1:15,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of gamma Actin in Whole cell lysate from HeLa (1, 2.5, 5, 15 and 50 µg) and mouse NIH3T3 cells (50 µg). Samples were incubated in gamma Actin polyclonal antibody (Product # PA1-16890) using a dilution of 0.04 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of gamma Actin in 1.0 mg/mL HeLa lysate. Samples were incubated in gamma Actin polyclonal antibody (Product # PA1-16890). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of gamma Actin in HeLa cells. Samples were incubated in gamma Actin polyclonal antibody (Product # PA1-16890) using a dilution of 1:50. Nuclei (Blue) were counterstained using Hoechst 33258.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of gamma Actin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with gamma Actin Polyclonal Antibody (Product # PA1-16890) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of gamma Actin in formalin-fixed paraffin-embedded section of human testicular seminoma. Samples were incubated in gamma Actin polyclonal antibody (Product # PA1-16890) using a dilution of 1:1,000. Detection: DAB.
