- NB600-533 - Novus Biologicals ACTG1 antibody

Submitted references c-Myb and C/EBPβ regulate OPN and other senescence-associated secretory phenotype factors.

Flanagan KC, Alspach E, Pazolli E, Parajuli S, Ren Q, Arthur LL, Tapia R, Stewart SA
Oncotarget 2018 Jan 2;9(1):21-36

Liquid chromatography-mass spectrometry-based quantitative proteomics analysis reveals chondroprotective effects of astragaloside IV in interleukin-1β-induced SW1353 chondrocyte-like cells.

Luo H, Yao L, Zhang Y, Li R
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Jul;91:796-802

Dual leucine zipper kinase regulates expression of axon guidance genes in mouse neuronal cells.

Blondeau A, Lucier JF, Matteau D, Dumont L, Rodrigue S, Jacques PÉ, Blouin R
Neural development 2016 Jul 28;11(1):13

Okazaki fragment processing-independent role for human Dna2 enzyme during DNA replication.

Duxin JP, Moore HR, Sidorova J, Karanja K, Honaker Y, Dao B, Piwnica-Worms H, Campbell JL, Monnat RJ Jr, Stewart SA
The Journal of biological chemistry 2012 Jun 22;287(26):21980-91

Human Dna2 is a nuclear and mitochondrial DNA maintenance protein.

Duxin JP, Dao B, Martinsson P, Rajala N, Guittat L, Campbell JL, Spelbrink JN, Stewart SA
Molecular and cellular biology 2009 Aug;29(15):4274-82

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Actin Gamma 1 Antibody [NB600-533] - Whole cell lysate from HeLa (1, 2.5, 5, 15 and 50 ug) and mouse NIH3T3 cells (50 ug), probed with diluted at 0.04 ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Actin Gamma 1 Antibody [NB600-533] - Actin Antibody [NB600-533]-Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: Actin Gamma 1 Antibody [NB600-533] - Simple Western lane view shows a specific band for Actin Gamma 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Actin Gamma 1 Antibody [NB600-533] - Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), Jurkat (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-Actin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Actin Gamma 1 Antibody [NB600-533] - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody NB600-533 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

NB600-533

Antibody data