PA5-43430

antibody from Invitrogen Antibodies

Targeting: MBOAT7 BB1, hMBOA-7, LENG4, LPLAT

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-43430

Provider

Invitrogen Antibodies

Product name

MBOAT7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: WWLAQYIYKS APARSYVLRS AWTMLLSAYW HGLHPGYYLS FLTIPLCLAA

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), MCF7 (Lane 2), SK-BR-3 (Lane 3), tissue extracts of Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Skeletal Muscle (Lane 6), Mouse Ovary (Lane 7) and Mouse Pancreas (Lane 8). The blot was probed with Anti-MBOAT7 Polyclonal Antibody (Product # PA5-43430, 0.2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 60 kDa band corresponding to MBOAT7 was observed across cell lines and tissues tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human brain cells using an anti-LENG4 polyclonal antibody (Product # PA5-43430).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of MBOAT7 was achieved by transfecting Hep G2 cells with MBOAT7 specific siRNAs (Silencer® select Product # s35614). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the MBOAT7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with MBOAT7 Polyclonal Antibody (Product # PA5-43430, 1µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MBOAT7.