Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-29952 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal ZNF202 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ZNF202 Antibody [NBP2-29952] - Analysis of (Center) (NBP2-29952) in HepG2 cell line lysates (35ug/lane). ZNF202 (arrow) was detected using the purified Pab.
Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunocytochemistry/Immunofluorescence: ZNF202 Antibody [NBP2-29952] - Fluorescent confocal image of MDA-MB231 cell stained with (Center)(NBP2-29952). MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF202 primary antibody (1:25, 1 h at 37). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ZNF202 immunoreactivity is localized to nucleus significantly.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ZNF202 Antibody [NBP2-29952] - Confocal immunofluorescent analysis of (Center) (NBP2-29952) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: ZNF202 Antibody [NBP2-29952] - Breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ZNF202 Antibody [NBP2-29952] - Analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.