Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 100-101-208 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-101-208, RRID:AB_2237313
- Product name
- Anti-PLASMINOGEN (Human Plasma) (GOAT) Antibody - 100-101-208
- Antibody type
- Polyclonal
- Vial size
- 2 µl
Submitted references Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue.
Shams F, Oldfield NJ, Lai SK, Tunio SA, Wooldridge KG, Turner DP
MicrobiologyOpen 2016 Apr;5(2):340-50
MicrobiologyOpen 2016 Apr;5(2):340-50
No comments: Submit comment
Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Rockland Goat anti Plasminogen antibody (200-101-208 lot 6571) was used to detect Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
- Validation comment
- Western Blot
