AF2325

antibody from R&D Systems

Targeting: RARRES2 HP10433, TIG2

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2325

Provider

R&D Systems

Product name

Mouse Chemerin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse Chemerin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human Chemerin is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9DD06


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Pro- and Anti-Inflammatory Role of ChemR23 Signaling in Pollutant-Induced Inflammatory Lung Responses.

Provoost S, De Grove KC, Fraser GL, Lannoy VJ, Tournoy KG, Brusselle GG, Maes T, Joos GF
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1882-90

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Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia.

Klose R, Krzywinska E, Castells M, Gotthardt D, Putz EM, Kantari-Mimoun C, Chikdene N, Meinecke AK, Schrödter K, Helfrich I, Fandrey J, Sexl V, Stockmann C
Nature communications 2016 Aug 19;7:12528

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The expression and regulation of chemerin in the epidermis.

Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel BA, Cichy J
PloS one 2015;10(2):e0117830

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Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis.

Muruganandan S, Dranse HJ, Rourke JL, McMullen NM, Sinal CJ
Stem cells (Dayton, Ohio) 2013 Oct;31(10):2172-82

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The role of ChemR23 in the induction and resolution of cigarette smoke-induced inflammation.

Demoor T, Bracke KR, Dupont LL, Plantinga M, Bondue B, Roy MO, Lannoy V, Lambrecht BN, Brusselle GG, Joos GF
Journal of immunology (Baltimore, Md. : 1950) 2011 May 1;186(9):5457-67

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Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.

Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ
The Journal of biological chemistry 2011 Jul 8;286(27):23982-95

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Chemerin peptides promote phagocytosis in a ChemR23- and Syk-dependent manner.

Cash JL, Christian AR, Greaves DR
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5315-24

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Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.

Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6489-99

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by Chemerin and Neutralization by Mouse Chemerin Antibody. Recombinant Mouse Chemerin (Catalog # 2325-CM) chemo-attracts the BaF3 mouse pro-B cell line transfected with human Chem R23 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Chemerin (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Chemerin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2325). The ND₅₀ is typically 3-9 µg/mL.