PAB29452

antibody from Abnova Corporation

Targeting: KPNA1 IPOA5, NPI-1, RCH2, SRP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29452

Provider

Abnova Corporation

Product name

KPNA1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human KPNA1.

Antigen sequence

MEMAPGGVITSDMIEMIFSKSPEQQLSATQK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp with KPNA1 polyclonal antibody (Cat # PAB29452) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with KPNA1 polyclonal antibody (Cat # PAB29452) at 1-4 ug/mL concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with KPNA1 polyclonal antibody (Cat # PAB29452) shows moderate nuclear positivity in Purkinje cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)