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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00003836-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003836-M01, RRID:AB_540073
- Product name
- Mouse Monoclonal Importin alpha 5/KPNA1/SRP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. KPNA1 - karyopherin alpha 1 (importin alpha 5)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references BIG3 Inhibits the Estrogen-Dependent Nuclear Translocation of PHB2 via Multiple Karyopherin-Alpha Proteins in Breast Cancer Cells.
Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box.
Distinct roles for classical nuclear import receptors in the growth of multinucleated muscle cells.
Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery.
Nuclear import impairment causes cytoplasmic trans-activation response DNA-binding protein accumulation and is associated with frontotemporal lobar degeneration.
Prohibitin inhibits tumor necrosis factor alpha-induced nuclear factor-kappa B nuclear translocation via the novel mechanism of decreasing importin alpha3 expression.
Kim NH, Yoshimaru T, Chen YA, Matsuo T, Komatsu M, Miyoshi Y, Tanaka E, Sasa M, Mizuguchi K, Katagiri T
PloS one 2015;10(6):e0127707
PloS one 2015;10(6):e0127707
Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box.
Moriyama T, Sangel P, Yamaguchi H, Obuse C, Miyamoto Y, Oka M, Yoneda Y
Biochemical and biophysical research communications 2015 Jul 3;462(3):201-7
Biochemical and biophysical research communications 2015 Jul 3;462(3):201-7
Distinct roles for classical nuclear import receptors in the growth of multinucleated muscle cells.
Hall MN, Griffin CA, Simionescu A, Corbett AH, Pavlath GK
Developmental biology 2011 Sep 1;357(1):248-58
Developmental biology 2011 Sep 1;357(1):248-58
Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery.
Moriyama T, Nagai M, Oka M, Ikawa M, Okabe M, Yoneda Y
The FEBS journal 2011 May;278(9):1561-72
The FEBS journal 2011 May;278(9):1561-72
Nuclear import impairment causes cytoplasmic trans-activation response DNA-binding protein accumulation and is associated with frontotemporal lobar degeneration.
Nishimura AL, Zupunski V, Troakes C, Kathe C, Fratta P, Howell M, Gallo JM, Hortobágyi T, Shaw CE, Rogelj B
Brain : a journal of neurology 2010 Jun;133(Pt 6):1763-71
Brain : a journal of neurology 2010 Jun;133(Pt 6):1763-71
Prohibitin inhibits tumor necrosis factor alpha-induced nuclear factor-kappa B nuclear translocation via the novel mechanism of decreasing importin alpha3 expression.
Theiss AL, Jenkins AK, Okoro NI, Klapproth JM, Merlin D, Sitaraman SV
Molecular biology of the cell 2009 Oct;20(20):4412-23
Molecular biology of the cell 2009 Oct;20(20):4412-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - Analysis of KPNA1 expression in transfected 293T cell line by KPNA1 monoclonal antibody (M01), clone 2A4-1B5.Lane 1: KPNA1 transfected lysate(60.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - KPNA1 monoclonal antibody (M01), clone 2A4-1B5 Analysis of KPNA1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - Detection limit for recombinant GST tagged KPNA1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - Analysis of monoclonal antibody to KPNA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.
