18137-1-AP

antibody from Invitrogen Antibodies

Targeting: KPNA1 IPOA5, NPI-1, RCH2, SRP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18137-1-AP

Provider

Invitrogen Antibodies

Product name

importin alpha 5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SLNPDEMRR RREEEGLQLR KQKREEQLFK RRNVATAEEE TEEEVMSDGG FHEAQINNME MAPGGVITSD MIEMIFSKSP EQQLSATQKF RKLLSKEPNP PIDEVISTPG VVARFVEFLK RKENCTLQFE SAWVLTNIAS GNSLQTRIVI QAGAVPIFIE LLSSEFEDVQ EQAVWALGNI AGDSTMCRDY VLDCNILPPL LQLFSKQNRL TMTRNAVWAL SNLCRGKSPP PEFAKVSPCL NVLSWLLFVS DTDVLA (18-272 aa encoded by BC002374)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 18137-1-AP (importin α5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 18137-1-AP (importin α5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using KPNA1 antibody 18137-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using KPNA1 antibody 18137-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 18137-1-AP (importin α5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 18137-1-AP (importin α5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-importin α5 (IP:18137-1-AP, 4ug; Detection:18137-1-AP 1:1000) with HEK-293 cells lysate 1480ug.