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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX103250 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103250, RRID:AB_1949660
- Product name
- Annexin V antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Therapeutic Challenge with a CDK 4/6 Inhibitor Induces an RB-Dependent SMAC-Mediated Apoptotic Response in Non-Small Cell Lung Cancer.
The Neuroprotective Role of MiR-124-3p in a 6-Hydroxydopamine-Induced Cell Model of Parkinson's Disease via the Regulation of ANAX5.
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina.
Thangavel C, Boopathi E, Liu Y, McNair C, Haber A, Perepelyuk M, Bhardwaj A, Addya S, Ertel A, Shoyele S, Birbe R, Salvino JM, Dicker AP, Knudsen KE, Den RB
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Mar 15;24(6):1402-1414
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Mar 15;24(6):1402-1414
The Neuroprotective Role of MiR-124-3p in a 6-Hydroxydopamine-Induced Cell Model of Parkinson's Disease via the Regulation of ANAX5.
Dong RF, Zhang B, Tai LW, Liu HM, Shi FK, Liu NN
Journal of cellular biochemistry 2018 Jan;119(1):269-277
Journal of cellular biochemistry 2018 Jan;119(1):269-277
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Chen JY, Chou HC, Chen YH, Chan HL
The Journal of nutritional biochemistry 2013 Nov;24(11):1889-910
The Journal of nutritional biochemistry 2013 Nov;24(11):1889-910
Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina.
Ruggiero L, Connor MP, Chen J, Langen R, Finnemann SC
Proceedings of the National Academy of Sciences of the United States of America 2012 May 22;109(21):8145-8
Proceedings of the National Academy of Sciences of the United States of America 2012 May 22;109(21):8145-8
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:HeLa S3(GTX14654)B:Hep G2(GTX27900)12% SDS PAGEGTX103250 diluted at 1:2000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A:NIH-3T310% SDS PAGE GTX103250 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Annexin V antibody detects ANXA5 protein by Western blot analysis.A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10 % SDS-PAGEAnnexin V antibody (GTX103250) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Annexin V antibody detects Annexin V protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat2 whole cell lysate/extract10% SDS-PAGEAnnexin V antibody (GTX103250) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Annexin V antibody detects Annexin V protein by western blot analysis.A. 30 ?g A431 whole cell lysate/extract B. 30 ?g HeLa whole cell lysate/extract C. 30 ?g HepG2 whole cell lysate/extract10% SDS-PAGEAnnexin V antibody (GTX103250) dilution: 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Annexin V antibody detects Annexin V protein by western blot analysis.A. 30 ?g GL261 whole cell lysate/extract B. 30 ?g C8D30 whole cell lysate/extract C. 30 ?g NIH-3T3 whole cell lysate/extract D. 30 ?g Raw 264.7 whole cell lysate/extract10% SDS-PAGEAnnexin V antibody (GTX103250) dilution: 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Annexin V knockout (KO) 293T cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody (GTX103250) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin V(GTX103250) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Annexin V antibody detects Annexin V protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Annexin V antibody (GTX103250) diluted at 1:500.
