11060-1-AP
antibody from Invitrogen Antibodies
Targeting: ANXA5
ANX5, ENX2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [10]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 11060-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin V Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.32 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human lung tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pig liver tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pig heart tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 11060-1-AP (Annexin V antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen tissue slide using 11060-1-AP ( Annexin V antibody at dilution of 1:400 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen tissue slide using 11060-1-AP ( Annexin V antibody at dilution of 1:400 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug Annexin V antibody (11060-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Annexin V (IP:11060-1-AP, 3ug; Detection:11060-1-AP 1:500) with HeLa cells lysate 3000ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Annexin V (IP:11060-1-AP, 4 ug; Detection:11060-1-AP 1:600) with mouse liver tissue lysate 7000 ug.