MA5-24146

antibody from Invitrogen Antibodies

Targeting: ESAM W117m

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24146

Provider

Invitrogen Antibodies

Product name

ESAM Monoclonal Antibody (408519)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross-reactivity with recombinant mouse ESAM is observed.

Antibody clone number

408519

Concentration

0.5 mg/mL

References

Submitted references

A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils.

Janela B, Patel AA, Lau MC, Goh CC, Msallam R, Kong WT, Fehlings M, Hubert S, Lum J, Simoni Y, Malleret B, Zolezzi F, Chen J, Poidinger M, Satpathy AT, Briseno C, Wohn C, Malissen B, Murphy KM, Maini AA, Vanhoutte L, Guilliams M, Vial E, Hennequin L, Newell E, Ng LG, Musette P, Yona S, Hacini-Rachinel F, Ginhoux F
Immunity 2019 Apr 16;50(4):1069-1083.e8

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of ESAM was detected in immersion fixed HUVEC human umbilical vein endothelial cells using mouse Anti-human ESAM Monoclonal Antibody (Product # MA5-24146) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (yello and counterstained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HUVEC human umbilical vein endothelial cells were stained with mouse Anti-human ESAM Monoclonal (Product # MA5-24146, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody.