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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX101948 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101948, RRID:AB_1951217
- Product name
- PHGDH antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX101948 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 £gg of whole cell lysate)A:293TB:A431(GTX27909)C:HeLa S3(GTX14654)7.5% SDS PAGEGTX101948 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using PHGDH(GTX101948) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 £gg of PHGDH antibody [N1N2], N-term (GTX101948).Western blot analysis was performed using PHGDH antibody [N1N2], N-term (GTX101948).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH(GTX101948) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PHGDH antibody [N1N2], N-term detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PHGDH antibody [N1N2], N-term detects PHGDH protein by immunohistochemical analysis.Sample: Frozen-sectioned rat E13.5 brain.Green: PHGDH stained by PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
