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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX101949 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101949, RRID:AB_11167012
- Product name
- PHGDH antibody [N3C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE GTX101949 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using PHGDH(GTX101949) antibody at 1:250 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PHGDH antibody [N3C2], Internal detects PHGDH protein by immunohistochemical analysis.Sample: Frozen-sectioned rat E13.5 brain.Green: PHGDH stained by PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
