Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- H00026227-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00026227-M01, RRID:AB_546991
- Product name
- Mouse Monoclonal PHGDH Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PHGDH - phosphoglycerate dehydrogenase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Enhanced serine production by bone metastatic breast cancer cells stimulates osteoclastogenesis.
Pollari S, Käkönen SM, Edgren H, Wolf M, Kohonen P, Sara H, Guise T, Nees M, Kallioniemi O
Breast cancer research and treatment 2011 Jan;125(2):421-30
Breast cancer research and treatment 2011 Jan;125(2):421-30
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody (M01), clone 4A3-1D6.Lane 1: PHGDH transfected lysate(56.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6 Analysis of PHGDH expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 1 ~ 10 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.