Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00026227-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00026227-D01, RRID:AB_10986786
- Product name
- PHGDH MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human PHGDH protein.
- Antigen sequence
MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQN
LSKEELIAELQDCEGLIVRSATKVTADVINAAEKL
QVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLS
AAELTCGMIMCLARQIPQATASMKDGKWERKKFMG
TELNGKTLGILGLGRIGREVATRMQSFGMKTIGYD
PIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLL
PSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGAL
LRALQSGQCAGAALDVFTEEPPRDRALVDHENVIS
CPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGV
VNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSP
KGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQ
ADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGE
CLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRP
EVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGV
RLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHV
TEAFQFHF- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PHGDH MaxPab rabbit polyclonal antibody. Western Blot analysis of PHGDH expression in human kidney.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PHGDH expression in transfected 293T cell line (H00026227-T02) by PHGDH MaxPab polyclonal antibody.Lane 1: PHGDH transfected lysate(56.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PHGDH transfected lysate using anti-PHGDH MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PHGDH purified MaxPab mouse polyclonal antibody (B01P) (H00026227-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol