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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-27578 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHGDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, 293T, A431, HeLaS3, mouse brain, SK-N-DZ.
- Concentration
- 0.87 mg/mL
Submitted references Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.
Zhang Y, Yu H, Zhang J, Gao H, Wang S, Li S, Wei P, Liang J, Yu G, Wang X, Li X, Li D, Yang W
The Journal of clinical investigation 2021 Nov 1;131(21)
The Journal of clinical investigation 2021 Nov 1;131(21)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PHGDH Polyclonal Antibody (Product # PA5-27578). Sample (30 µg of whole cell lysate). A: 293T. B: A431. C: HeLa S3. 7.5% SDS PAGE. PHGDH Polyclonal Antibody (Product # PA5-27578) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PHGDH Polyclonal Antibody (Product # PA5-27578). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. PHGDH Polyclonal Antibody (Product # PA5-27578) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-PHGDH Polyclonal Antibody (Product # PA5-27578) and a 57 kDa band corresponding to D-3-phosphoglycerate dehydrogenase was observed across cell lines and tissue tested except in A549, MDA-MB-231, and SK-O-V3 which are reported to be low to negative for PHGDH expression. An additional uncharacterized band (*) was observed across the panel. Whole-cell extracts (30 µg lysate) of NTERA-2 cl.D1 (Lane 1), A549 (Lane 2), HCT 116 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), LNCaP (Lane 6), SK-O-V3 (Lane 7), Raji (Lane 8) and Mouse Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PHGDH in methanol-fixed HeLa cells using a PHGDH polyclonal antibody (Product # PA5-27578) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of PHGDH was performed in frozen-sectioned rat E13.5 brain tissue using PHGDH Polyclonal Antibody (Product # PA5-27578) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH (Product # PA5-27578) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PHGDH Polyclonal Antibody detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. PHGDH Polyclonal Antibody (Product # PA5-27578) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PHGDH was performed in 293T whole cell extracts using 5 µg of PHGDH Polyclonal Antibody (Product # PA5-27578). Samples were transferred to a membrane and probed with PHGDH Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
