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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- TA328601 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-hKV11.1 (HERG)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-hKV11.1 (HERG)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KCNH2
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Kv11.1 expressing HEK-293 cells: 1. Anti-hKv11.1 (HERG) antibody, (1:400). 2. Anti-hKv11.1 (HERG) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Kv11.1 in HEK-293 transfected cells Immunocytochemical staining of fixed and permeabilized Kv11.1 transfected HEK-293 cells. Cells were stained with Anti-hKv11.1 (HERG) antibody, followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (Red). Almost all transfected cells are stained positive for the Kv11.1. Arrows indicate cells that do not express the channel.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of Kv11.1 expressing HEK-293 cells: 1. Cell lysate. 2. Lysate + protein A beads + Anti- hKv11.1 (HERG) antibody. 3. Cell lysate + protein A beads + Anti-Kv11.1 (erg1) antibody. 4. Cell lysate + protein A beads + Anti-Kv11.1 (HERG) (extracellular) antibody . 5. Cell lysate + protein A beads + pre-immune rabbit serum. Red arrow indicates Kv11.1 while the black arrow shows the IgG heavy chain. Immunoblot was performed with the Anti- hKv11.1 (HERG) antibody.
- Validation comment
- IP
