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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA328602 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-KV11.1 (HERG) (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-KV11.1 (HERG) (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KCNH2
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Kv11.1 transfected HEK-293 cells: 1. Anti-Kv11.1 (HERG) (extracellular) antibody, (1:200). 2. Anti-Kv11.1 (HERG) (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ICC staining of live intact HEK-293 transfected cells.Cells were stained with Anti-Kv11.1 (HERG) (extracellular) antibody , (1:25) (left panel) or with Kv11.1 (HERG) (extracellular) antibody preincubated with the control peptide antigen (right panel), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei of the living cells were stained with the cell permeable dye Hoechst 33342 (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of Kv11.1 expressing HEK-293 cells: Cell lysate. Lysate + protein A beads + Anti-Kv11.1 (HERG) (extracellular) antibody. Lysate + protein A beads + Anti-Kv11.1 (erg1) antibody. Lysate + protein A beads + Anti-hKv11.1 (HERG) antibody . Lysate + protein A beads + pre-immune rabbit serum. Red arrow indicates Kv11.1 while the black arrow shows the IgG heavy chain. Immunoblot was performed with Anti-Kv11.1 (HERG) (extracellular) antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Kv11.1 in rat heart Immunohistochemical staining of rat heart using Anti-Kv11.1 (HERG) (extracellular) antibody. A. Transversal section of the atrium wall; note that arterial smooth muscle fibers were not stained (green arrow). B. Longitudinal section of the myocardium. C. Section showing myocardium and endocardium (red arrow). DAB product is brown and the counterstain is cresyl violet.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Indirect flow cytometry analysis of human chronic myelogenous leukemia (K562) cells: green line, Unstained cells + goat-anti-rabbit-FITC. black line, Cells + Anti-KV11.1 (HERG) (extracellular) antibody + goat-anti-rabbit-FITC.
- Validation comment
- FC
