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LearnAPC-109-25UL
antibody from Invitrogen Antibodies
Targeting: KCNH2
erg1, HERG, Kv11.1, LQT2
Western blot
Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- APC-109-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KCNH2 (HERG) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.6 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of KV11.1 (HERG) in HEK-293 transfected cells - Cell surface detection of KV11.1 in live intact HEK-293 transfected cells. Cellswere stained with Anti-KCNH2 (HERG) (extracellular) Antibody (#APC-109), (1:25) (left panel) or with Anti-KCNH2 (HERG) (extracellular) Antibody preincubated with the blocking peptide (right panel), followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody (red). Nuclei of the living cells were stained with the cell permeable dye Hoechst 33342 (blue). Note that not all cells are stained, indicating that not all the KV11.1 channels are expressed at the cell membrane at the moment of staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of KV11.1 (HERG) in rat heart - Immunohistochemical staining of rat heart using Anti-KCNH2 (HERG) (extracellular) Antibody (#APC-109). A. Transversal section of the atrium wall; note that arterial smooth muscle fibers were not stained (green arrow).B. Longitudinal section of the myocardium. C. Section showing myocardium and endocardium (red arrow). DAB product is brown and the counterstain is cresyl violet.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of KV11.1 (HERG) by indirect flow cytometry in live intact human chronic myelogenous leukemia (K562) cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-KCNH2 (HERG) (extracellular) Antibody (#APC-109), (2.5μg) + goat- Anti-rabbit-FITC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of KV11.1-expressing HEK-293 cells: - 1. Cell lysate. 2. Cell lysate + protein A beads + Anti-KCNH2 (HERG) (extracellular) Antibody (#APC-109).3. Cell lysate + protein A beads + Anti-KCNH2 (erg1) Antibody (#APC-016).4. Cell lysate + protein A beads + Anti-KCNH2 (HERG) Antibody (#APC-062).5. Cell lysate + protein A beads + pre-immune rabbit serum. Red arrow indicates KV11.1 while the black arrow shows the IgG heavy chain. Immunoblot was performed with Anti-KCNH2 (HERG) (extracellular) Antibody .
