PA5-77776

antibody from Invitrogen Antibodies

Targeting: ATG9B APG9L2, FLJ14885, NOS3AS, SONE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-77776

Provider

Invitrogen Antibodies

Product name

ATG9B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATG9B in rat brain cell lysates with 15 µg of sample. The sample was incubated with ATG9B polyclonal antibody (Product # PA5-77776) using a dilution of 1:1000 (16 hours at 4°C), and followed by Goat Anti-Rabbit HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder (MW), Lane 2: Rat Brain cell lysates.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATG9B in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ATG9B polyclonal antibody (Product # PA5-77776) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) ATG9B Antibody D) Merged image. Magnification: 60x.