13108-1-AP

antibody from Proteintech Group

Targeting: PYCR1 P5C

Provider product page for 13108-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [46]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 13108-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13108-1-AP, RRID:AB_2174878
Product name: PYCR1 antibody
Antibody type: Polyclonal
Description: KD/KO validated PYCR1 antibody (Cat. #13108-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PYCR1 protein structure - 13108-1-AP shown in red.

Submitted references Pro-tumorigenic activity of PYCR1 in gastric cancer through regulating the PI3K/AKT signaling.

Xiao H, Huang J, Wu H, Li Y, Wang Y
Heliyon 2024 Mar 15;10(5):e26883

Proline restores mitochondrial function and reverses aging hallmarks in senescent cells.

Choudhury D, Rong N, Senthil Kumar HV, Swedick S, Samuel RZ, Mehrotra P, Toftegaard J, Rajabian N, Thiyagarajan R, Podder AK, Wu Y, Shahini S, Seldeen KL, Troen B, Lei P, Andreadis ST
Cell reports 2024 Feb 27;43(2):113738

Loss of attachment promotes proline accumulation and excretion in cancer cells.

Pilley SE, Hennequart M, Vandekeere A, Blagih J, Legrave NM, Fendt SM, Vousden KH, Labuschagne CF
Science advances 2023 Sep 8;9(36):eadh2023

IGF1R-phosphorylated PYCR1 facilitates ELK4 transcriptional activity and sustains tumor growth under hypoxia.

Zheng K, Sha N, Hou G, Leng Z, Zhao Q, Zhang L, He L, Xu M, Jiang Y, Chen T
Nature communications 2023 Sep 30;14(1):6117

Mechanical stiffness promotes skin fibrosis through Piezo1-mediated arginine and proline metabolism.

He J, Fang B, Shan S, Li Q
Cell death discovery 2023 Sep 26;9(1):354

Metabolomic and transcriptomic studies of improvements in myocardial infarction due to Pycr1 deletion.

Xue Z, Pan Y, Kong X, Zhang J, Wu D, Zhou B
Journal of cellular and molecular medicine 2023 Jan;27(1):89-100

SLC25A51 promotes tumor growth through sustaining mitochondria acetylation homeostasis and proline biogenesis.

Li Y, Bie J, Zhao L, Song C, Zhang T, Li M, Yang C, Luo J
Cell death and differentiation 2023 Aug;30(8):1916-1930

Proline is increased in allergic asthma and promotes airway remodeling.

Xu T, Wu Z, Yuan Q, Zhang X, Liu Y, Wu C, Song M, Wu J, Jiang J, Wang Z, Chen Z, Zhang M, Huang M, Ji N
JCI insight 2023 Aug 22;8(16)

Shikonin induces apoptosis and autophagy via downregulation of pyrroline-5-carboxylate reductase1 in hepatocellular carcinoma cells.

Zhang J, Shang L, Jiang W, Wu W
Bioengineered 2022 Mar;13(3):7904-7918

Proline synthesis through PYCR1 is required to support cancer cell proliferation and survival in oxygen-limiting conditions.

Westbrook RL, Bridges E, Roberts J, Escribano-Gonzalez C, Eales KL, Vettore LA, Walker PD, Vera-Siguenza E, Rana H, Cuozzo F, Eskla KL, Vellama H, Shaaban A, Nixon C, Luuk H, Lavery GG, Hodson DJ, Harris AL, Tennant DA
Cell reports 2022 Feb 1;38(5):110320

Epigenetic modification facilitates proline synthase PYCR1 aberrant expression in gastric cancer.

Xiao S, Yao X, Ye J, Tian X, Yin Z, Zhou L
Biochimica et biophysica acta. Gene regulatory mechanisms 2022 Aug;1865(6):194829

Deciphering the effects of PYCR1 on cell function and its associated mechanism in hepatocellular carcinoma.

Xu Y, Zuo W, Wang X, Zhang Q, Gan X, Tan N, Jia W, Liu J, Li Z, Zhou B, Zhao D, Xie Z, Tan Y, Zheng S, Liu C, Li H, Chen Z, Yang X, Huang Z
International journal of biological sciences 2021;17(9):2223-2239

Knockdown of PYCR1 suppressed the malignant phenotype of human hepatocellular carcinoma cells via inhibiting the AKT pathway activation.

Guo J, Cheng X, Tian Y, Li B, Zhang X, Gao X, An Y
Reproductive biology 2021 Sep;21(3):100534

Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.

Chen Z, Wang C, Feng X, Nie L, Tang M, Zhang H, Xiong Y, Swisher SK, Srivastava M, Chen J
The EMBO journal 2021 Sep 1;40(17):e107776

Discovery of key genes as novel biomarkers specifically associated with HPV-negative cervical cancer.

Liu Y, Xu Y, Jiang W, Ji H, Wang ZW, Zhu X
Molecular therapy. Methods & clinical development 2021 Jun 11;21:492-506

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.

Zhang P, Wang J, Luo W, Yuan J, Cui C, Guo L, Wu C
American journal of respiratory cell and molecular biology 2021 Jul;65(1):54-69

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Jones B, Balasubramaniam M, Lebowitz JJ, Taylor A, Villalta F, Khoshbouei H, Grueter C, Grueter B, Dash C, Pandhare J
Scientific reports 2021 Jan 14;11(1):1422

PINCH-1 promotes Δ(1)-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Cui C, Wang J, Guo L, Wu C
Amino acids 2021 Dec;53(12):1875-1890

Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.

Tran DH, Kesavan R, Rion H, Soflaee MH, Solmonson A, Bezwada D, Vu HS, Cai F, Phillips JA 3rd, DeBerardinis RJ, Hoxhaj G
Nature metabolism 2021 Apr;3(4):571-585

Proteomic patterns associated with response to breast cancer neoadjuvant treatment.

Shenoy A, Belugali Nataraj N, Perry G, Loayza Puch F, Nagel R, Marin I, Balint N, Bossel N, Pavlovsky A, Barshack I, Kaufman B, Agami R, Yarden Y, Dadiani M, Geiger T
Molecular systems biology 2020 Sep;16(9):e9443

PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.

Guo L, Cui C, Wang J, Yuan J, Yang Q, Zhang P, Su W, Bao R, Ran J, Wu C
Nature communications 2020 Oct 1;11(1):4913

Cancer progression is mediated by proline catabolism in non-small cell lung cancer.

Liu Y, Mao C, Wang M, Liu N, Ouyang L, Liu S, Tang H, Cao Y, Liu S, Wang X, Xiao D, Chen C, Shi Y, Yan Q, Tao Y
Oncogene 2020 Mar;39(11):2358-2376

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Escande-Beillard N, Loh A, Saleem SN, Kanata K, Hashimoto Y, Altunoglu U, Metoska A, Grandjean J, Ng FM, Pomp O, Baburajendran N, Wong J, Hill J, Beillard E, Cozzone P, Zaki M, Kayserili H, Hamada H, Shiratori H, Reversade B
Neuron 2020 Jul 8;107(1):82-94.e6

Pyrroline-5-carboxylate reductase 1 (PYCR1) upregulation contributes to gastric cancer progression and indicates poor survival outcome.

Xiao S, Li S, Yuan Z, Zhou L
Annals of translational medicine 2020 Aug;8(15):937

Proline improves cardiac remodeling following myocardial infarction and attenuates cardiomyocyte apoptosis via redox regulation.

Wang J, Xue Z, Lin J, Wang Y, Ying H, Lv Q, Hua C, Wang M, Chen S, Zhou B
Biochemical pharmacology 2020 Aug;178:114065

Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.

Ding Z, Ericksen RE, Escande-Beillard N, Lee QY, Loh A, Denil S, Steckel M, Haegebarth A, Wai Ho TS, Chow P, Toh HC, Reversade B, Gruenewald S, Han W
Journal of hepatology 2020 Apr;72(4):725-735

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606

SIRT3 regulates cancer cell proliferation through deacetylation of PYCR1 in proline metabolism.

Chen S, Yang X, Yu M, Wang Z, Liu B, Liu M, Liu L, Ren M, Qi H, Zou J, Vucenik I, Zhu WG, Luo J
Neoplasia (New York, N.Y.) 2019 Jul;21(7):665-675

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Guo L, Cui C, Zhang K, Wang J, Wang Y, Lu Y, Chen K, Yuan J, Xiao G, Tang B, Sun Y, Wu C
Nature communications 2019 Feb 19;10(1):845

Pyrroline-5-carboxylate reductase 1 promotes cell proliferation via inhibiting apoptosis in human malignant melanoma.

Ye Y, Wu Y, Wang J
Cancer management and research 2018;10:6399-6407

Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.

Dogan SA, Cerutti R, Benincá C, Brea-Calvo G, Jacobs HT, Zeviani M, Szibor M, Viscomi C
Cell metabolism 2018 Nov 6;28(5):764-775.e5

Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.

Hollinshead KER, Munford H, Eales KL, Bardella C, Li C, Escribano-Gonzalez C, Thakker A, Nonnenmacher Y, Kluckova K, Jeeves M, Murren R, Cuozzo F, Ye D, Laurenti G, Zhu W, Hiller K, Hodson DJ, Hua W, Tomlinson IP, Ludwig C, Mao Y, Tennant DA
Cell reports 2018 Mar 20;22(12):3107-3114

A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder.

Huang YW, Chiang MF, Ho CS, Hung PL, Hsu MH, Lee TH, Chu LJ, Liu H, Tang P, Victor Ng W, Lin DS
Aging and disease 2018 Dec;9(6):1043-1057

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.

Kühl I, Miranda M, Atanassov I, Kuznetsova I, Hinze Y, Mourier A, Filipovska A, Larsson NG
eLife 2017 Nov 14;6

Sublethal endoplasmic reticulum stress caused by the mutation of immunoglobulin heavy chain-binding protein induces the synthesis of a mitochondrial protein, pyrroline-5-carboxylate reductase 1.

Jin H, Komita M, Koseki H, Aoe T
Cell stress & chaperones 2017 Jan;22(1):77-85

Clusterin and Pycr1 alterations associate with strain and model differences in susceptibility to experimental pancreatitis.

Iyer S, Park MJ, Moons D, Kwan R, Liao J, Liu L, Omary MB
Biochemical and biophysical research communications 2017 Jan 22;482(4):1346-1352

PYCR2 Mutations cause a lethal syndrome of microcephaly and failure to thrive.

Zaki MS, Bhat G, Sultan T, Issa M, Jung HJ, Dikoglu E, Selim L, G Mahmoud I, Abdel-Hamid MS, Abdel-Salam G, Marin-Valencia I, Gleeson JG
Annals of neurology 2016 Jul;80(1):59-70

Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.

Loayza-Puch F, Rooijers K, Buil LC, Zijlstra J, Oude Vrielink JF, Lopes R, Ugalde AP, van Breugel P, Hofland I, Wesseling J, van Tellingen O, Bex A, Agami R
Nature 2016 Feb 25;530(7591):490-4

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Liu W, Hancock CN, Fischer JW, Harman M, Phang JM
Scientific reports 2015 Nov 24;5:17206

Understanding pyrroline-5-carboxylate synthetase deficiency: clinical, molecular, functional, and expression studies, structure-based analysis, and novel therapy with arginine.

Martinelli D, Häberle J, Rubio V, Giunta C, Hausser I, Carrozzo R, Gougeard N, Marco-Marín C, Goffredo BM, Meschini MC, Bevivino E, Boenzi S, Colafati GS, Brancati F, Baumgartner MR, Dionisi-Vici C
Journal of inherited metabolic disease 2012 Sep;35(5):761-76

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Liu W, Le A, Hancock C, Lane AN, Dang CV, Fan TW, Phang JM
Proceedings of the National Academy of Sciences of the United States of America 2012 Jun 5;109(23):8983-8

A novel mutation in PYCR1 causes an autosomal recessive cutis laxa with premature aging features in a family.

Lin DS, Yeung CY, Liu HL, Ho CS, Shu CH, Chuang CK, Huang YW, Wu TY, Huang ZD, Jian YR, Lin SP
American journal of medical genetics. Part A 2011 Jun;155A(6):1285-9

Compound heterozygous mutations in PYCR1 further expand the phenotypic spectrum of De Barsy syndrome.

Lin DS, Chang JH, Liu HL, Wei CH, Yeung CY, Ho CS, Shu CH, Chiang MF, Chuang CK, Huang YW, Wu TY, Jian YR, Huang ZD, Lin SP
American journal of medical genetics. Part A 2011 Dec;155A(12):3095-9

Further expansion of the phenotypic spectrum associated with mutations in ALDH18A1, encoding Δ¹-pyrroline-5-carboxylate synthase (P5CS).

Skidmore DL, Chitayat D, Morgan T, Hinek A, Fischer B, Dimopoulou A, Somers G, Halliday W, Blaser S, Diambomba Y, Lemire EG, Kornak U, Robertson SP
American journal of medical genetics. Part A 2011 Aug;155A(8):1848-56

Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.

Possemato R, Marks KM, Shaul YD, Pacold ME, Kim D, Birsoy K, Sethumadhavan S, Woo HK, Jang HG, Jha AK, Chen WW, Barrett FG, Stransky N, Tsun ZY, Cowley GS, Barretina J, Kalaany NY, Hsu PP, Ottina K, Chan AM, Yuan B, Garraway LA, Root DE, Mino-Kenudson M, Brachtel EF, Driggers EM, Sabatini DM
Nature 2011 Aug 18;476(7360):346-50

Mutations in PYCR1 cause cutis laxa with progeroid features.

Reversade B, Escande-Beillard N, Dimopoulou A, Fischer B, Chng SC, Li Y, Shboul M, Tham PY, Kayserili H, Al-Gazali L, Shahwan M, Brancati F, Lee H, O'Connor BD, Schmidt-von Kegler M, Merriman B, Nelson SF, Masri A, Alkazaleh F, Guerra D, Ferrari P, Nanda A, Rajab A, Markie D, Gray M, Nelson J, Grix A, Sommer A, Savarirayan R, Janecke AR, Steichen E, Sillence D, Hausser I, Budde B, Nürnberg G, Nürnberg P, Seemann P, Kunkel D, Zambruno G, Dallapiccola B, Schuelke M, Robertson S, Hamamy H, Wollnik B, Van Maldergem L, Mundlos S, Kornak U
Nature genetics 2009 Sep;41(9):1016-21

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human brain tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP(PYCR1 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using PYCR1 antibody 13108-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 13108-1-AP(PYCR1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue