H00005831-B01P

antibody from Abnova Corporation

Targeting: PYCR1 P5C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005831-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005831-B01P, RRID:AB_1579444

Product name

PYCR1 purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human PYCR1 protein.

Antigen sequence

MSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSP
DMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAV
KPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIE
KKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTH
AQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSG
SGPAYAFTALDALADGGVKMGLPRRLAVRLGAQAL
LGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVL
ESGGFRSLLINAVEASCIRTRELQSMADQEQVSPA
AIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAP
AGKD


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Functional specialization in proline biosynthesis of melanoma.

De Ingeniis J, Ratnikov B, Richardson AD, Scott DA, Aza-Blanc P, De SK, Kazanov M, Pellecchia M, Ronai Z, Osterman AL, Smith JW
PloS one 2012;7(9):e45190

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PYCR1 MaxPab polyclonal antibody. Western Blot analysis of PYCR1 expression in U-2 OS.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PYCR1 expression in transfected 293T cell line (H00005831-T01) by PYCR1 MaxPab polyclonal antibody.Lane 1: PYCR1 transfected lysate(35.09 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to PYCR1 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol