Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00005831-B01P - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005831-B01P, RRID:AB_2174880
- Product name
- Mouse Polyclonal PYCR1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. PYCR1 - pyrroline-5-carboxylate reductase 1,
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Functional specialization in proline biosynthesis of melanoma.
De Ingeniis J, Ratnikov B, Richardson AD, Scott DA, Aza-Blanc P, De SK, Kazanov M, Pellecchia M, Ronai Z, Osterman AL, Smith JW
PloS one 2012;7(9):e45190
PloS one 2012;7(9):e45190
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYCR1 Antibody [H00005831-B01P] - Expression in U-2 OS.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYCR1 Antibody [H00005831-B01P] - Analysis of PYCR1 expression in transfected 293T cell line by PYCR1 polyclonal antibody. Lane 1: PYCR1 transfected lysate (35.09 kDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYCR1 Antibody [H00005831-B01P] - Pig liver extracts. Lane 1: 30 ug, Lane 2: 45 ug, Lane 3: 60 ug, Lane 4: secondary anti-mouse antibody only. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PYCR1 Antibody [H00005831-B01P] - Analysis of purified antibody to PYCR1 on HeLa cell (antibody concentration 10 ug/mL).