Explore
Validate
Learn
Western blot
Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00001027-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001027-M01, RRID:AB_537661
- Product name
- Mouse Monoclonal p27/Kip1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CDKN1B - cyclin-dependent kinase inhibitor 1B (p27, Kip1)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Increase of Intracellular Cyclic AMP by PDE4 Inhibitors Affects HepG2 Cell Cycle Progression and Survival.
Massimi M, Cardarelli S, Galli F, Giardi MF, Ragusa F, Panera N, Cinque B, Cifone MG, Biagioni S, Giorgi M
Journal of cellular biochemistry 2017 Jun;118(6):1401-1411
Journal of cellular biochemistry 2017 Jun;118(6):1401-1411
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Western Blot analysis of CDKN1B expression in HL-60 ( Cat # L014V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of CDKN1B expression in transfected 293T cell line by CDKN1B monoclonal antibody (M01), clone 4B4-E6.Lane 1: CDKN1B transfected lysate(22.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Detection limit for recombinant GST tagged CDKN1B is 10 ng/ml as a capture antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Detection limit for recombinant GST tagged CDKN1B is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of monoclonal antibody to CDKN1B on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of monoclonal antibody to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 5 ug/ml.
