HPA038771

antibody from Atlas Antibodies

Targeting: EYA4 CMD1J, DFNA10

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA038771

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA038771, RRID:AB_10671865

Product name

Anti-EYA4

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TGGENMTVLNTADWLLSCNTPSSATMSLLAVKTEP
LNSSETTATTGDGALDTFTGSVITSSGYSPRSA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines HeLa and MCF-7 using Anti-EYA4 antibody. Corresponding EYA4 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows distinct cytoplasmic positivity in myocytes.

Sample type

HUMAN