NBP1-41100

antibody from Novus Biologicals

Targeting: EYA4 CMD1J, DFNA10

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-41100

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-41100, RRID:AB_2262585

Product name

Rabbit Polyclonal EYA4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EYA4 Antibody [NBP1-41100] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EYA4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. EYA4 was also immunoprecipitated by rabbit anti-EYA4 antibody NBP1-41099, which recognizes an upstream epitope.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-41100] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: EYA4 Antibody [NBP1-41100] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-EYA4 used at a dilution of 1:200 (1ug/ml). Detection: DAB