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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PA5-17519 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAC1/RAC2/RAC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
Submitted references Phosphodiesterase 5 (PDE5) Is Highly Expressed in Cancer-Associated Fibroblasts and Enhances Breast Tumor Progression.
Catalano S, Panza S, Augimeri G, Giordano C, Malivindi R, Gelsomino L, Marsico S, Giordano F, Győrffy B, Bonofiglio D, Andò S, Barone I
Cancers 2019 Nov 6;11(11)
Cancers 2019 Nov 6;11(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), U-87 MG (Lane 4), 3T3-L1 (Lane 5) and NIH/3T3 (Lane 6). The blot was probed with Anti-RAC1/RAC2/RAC2 Polyclonal Antibody (Product # PA5-17519, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 20 kDa band corresponding to RAC1/RAC2/RAC3 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Rac1/2/3 in Jurkat cells using a Rac1/2/3 polyclonal antibody (Product # PA5-17519) (blue) compared to a nonspecific negative control antibody (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 PDE5 overexpression and fibroblast activation. ( A ) Immunoblotting for PDE5 expression in mouse embryonic fibroblasts (MEFs) stably transfected with pEGFP (enhanced-green-fluorescent-protein) vector (V) and pEGFP-PDE5A expression plasmid (PDE5 1 and PDE5 2). beta-Actin was used as a control for equal loading and transfer. Italicized numbers below blots represent the mean of the band optical density expressed as fold over V for PDE1 and PDE2. ( B ) MTT growth and ( C ) Trypan blue cell count assays in V, PDE5 1, and PDE5 2 stable clones under basal nonstimulated conditions at indicated times. ( D ) Wound healing assay in V, PDE5 1, and PDE5 2 stable clones with images captured at 0 (inset) and 12 h. Pictures are representative of three independent experiments. ( E ) Boyden chamber transmigration and ( F ) invasion assays in V, PDE5 1, and PDE5 2 stable clones under basal nonstimulated conditions. ( G ) Immunofluorescent staining of phalloidin staining of F-actin (stress fibers, red, upper panel) and alpha-SMA (lower panel) and in stable clones. 4',6-Diamidino-2-phenylindole (DAPI) staining was used for nuclei detection (inset). Pictures are representative of three independent experiments. Scale bar = 5 um. ( H ) Immunoblotting for N-cadherin, alpha-SMA, Rho A-C, Rac 1-3, and cdc42 expression levels in V, PDE5 1, and PDE5 2 stable clones. beta-Actin was used as a control for equal loading and transfer. Italicized numbers below blots represent the mean of the band op
