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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-31759 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCDC14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, U87-MG, SK-N-SH, SK-N-AS. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.
Meitinger F, Ohta M, Lee KY, Watanabe S, Davis RL, Anzola JV, Kabeche R, Jenkins DA, Shiau AK, Desai A, Oegema K
Nature 2020 Sep;585(7825):440-446
Nature 2020 Sep;585(7825):440-446
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CCDC14 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CCDC14 Polyclonal Antibody (Product # PA5-31759) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CCDC14 Polyclonal Antibody (Product # PA5-31759). Sample (30 µg of whole cell lysate). Lane A: A431. 7.5% SDS PAGE. CCDC14 Polyclonal Antibody (Product # PA5-31759) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CCDC14 in paraformaldehyde-fixed A431 cells using a CCDC14 polyclonal antibody (Product # PA5-31759) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CCDC14 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CCDC14 Polyclonal Antibody (Product # PA5-31759) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
