Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN498034 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-POU Class 3 Homeobox 3 (POU3F3) (Internal Region) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the internal region of the protein sequence according to NP_006227.1. Genename: POU3F3
- Description
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Antigen sequence
C-HMLSHAHQWVTAL
- Epitope
- Internal Region
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif.
Castro DS, Skowronska-Krawczyk D, Armant O, Donaldson IJ, Parras C, Hunt C, Critchley JA, Nguyen L, Gossler A, Göttgens B, Matter JM, Guillemot F
Developmental cell 2006 Dec;11(6):831-44
Developmental cell 2006 Dec;11(6):831-44
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP20121PU-N POU3F3 antibody staining of Mouse Spinal Cord lysate at 1 μg/ml (RIPA buffer, 35 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.