PA5-98666

antibody from Invitrogen Antibodies

Targeting: AHI1 FLJ20069, JBTS3, ORF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-98666

Provider

Invitrogen Antibodies

Product name

Jouberin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.54 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Jouberin using a Jouberin Polyclonal antibody (Product # PA5-98666). Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysate, U251 whole cell lysate. All lanes: AHI1 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 138 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Jouberin in paraffin embedded human endometrial cancer using a Jouberin polyclonal antibody (Product # PA5-98666) at a dilution of 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.