H00028960-M03

antibody from Abnova Corporation

Targeting: DCPS HINT-5, HINT5, HSL1, HSPC015

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00028960-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00028960-M03, RRID:AB_894061

Product name

DCPS monoclonal antibody (M03), clone 3E5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant DCPS.

Antigen sequence

MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNG
TCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNE
ASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQ
LQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHL
QKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQW
VYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQ
QQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNIL
HQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHF
TALGFEAPGSGVERAHLLAEVIENLECDPRHYQQR
TLTFALRADDPLLKLLQEAQQS


Isotype

IgG

Antibody clone number

3E5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DCPS monoclonal antibody (M03), clone 3E5. Western Blot analysis of DCPS expression in PC-12(Cat # L012V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DCPS is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol