Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB4349 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4349, RRID:AB_1578221
- Product name
- NUB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NUB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of the NEDD8 conjugation system by a splicing variant, NUB1L.
The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1.
NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression.
Tanaka T, Kawashima H, Yeh ET, Kamitani T
The Journal of biological chemistry 2003 Aug 29;278(35):32905-13
The Journal of biological chemistry 2003 Aug 29;278(35):32905-13
The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1.
Akey DT, Zhu X, Dyer M, Li A, Sorensen A, Blackshaw S, Fukuda-Kamitani T, Daiger SP, Craft CM, Kamitani T, Sohocki MM
Human molecular genetics 2002 Oct 15;11(22):2723-33
Human molecular genetics 2002 Oct 15;11(22):2723-33
NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression.
Kito K, Yeh ET, Kamitani T
The Journal of biological chemistry 2001 Jun 8;276(23):20603-9
The Journal of biological chemistry 2001 Jun 8;276(23):20603-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The NUB1 polyclonal antibody (Cat # PAB4349) is used in Western blot to detect NUB1 in mouse liver lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NUB1 polyclonal antibody (Cat # PAB4349) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)