RQ8328

antibody from NSJ Bioreagents

Targeting: NUB1 BS4, NUB1L, NYREN18

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ8328

Provider

NSJ Bioreagents

Product name

NUB1 Antibody / NEDD8 ultimate buster 1 / NYREN18

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Vial size

100 ug

Storage

After reconstitution, the NUB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human COLO-320, 3) human HepG2, 4) human K562, 5) human Raji, 6) monkey COS-7, 7) human MCF7, 8) human A549, 9) rat liver, 10) rat PC-12 and 11) mouse NIH 3T3 with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HeLa cells with NUB1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human adrenal adenoma tissue with NUB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human bladder urothelial carcinoma tissue with NUB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human ovarian cancer tissue with NUB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human K562 cells with NUB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NUB1 antibody.