HPA026962

antibody from Atlas Antibodies

Targeting: KCNJ1 Kir1.1, ROMK1

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA026962

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA026962, RRID:AB_10601934

Product name

Anti-KCNJ1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

STSATCQVRTSYVPEEVLWGYRFAPIVSKTKEGKY
RVDFHNFSKTVEVETPHCAMCLYNEKDVRARMKRG
YDNPNFILSEVNETD


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

WNK1-related Familial Hyperkalemic Hypertension results from an increased expression of L-WNK1 specifically in the distal nephron.

Vidal-Petiot E, Elvira-Matelot E, Mutig K, Soukaseum C, Baudrie V, Wu S, Cheval L, Huc E, Cambillau M, Bachmann S, Doucet A, Jeunemaitre X, Hadchouel J
Proceedings of the National Academy of Sciences of the United States of America 2013 Aug 27;110(35):14366-71

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human kidney and lymph node tissues using Anti-KCNJ1 antibody. Corresponding KCNJ1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows low expression as expected.

Sample type

HUMAN