NB100-1285

antibody from Novus Biologicals

Targeting: FOXK2 ILF, ILF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1285

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1285, RRID:AB_10002346

Product name

Goat Polyclonal ILF1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

FOXK2 transcription factor is a novel G/T-mismatch DNA binding protein.

Fujii Y, Nakamura M
Journal of biochemistry 2010 May;147(5):705-9

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Characterization and chromosomal mapping of the gene encoding the cellular DNA binding protein ILF.

Li C, Lusis AJ, Sparkes R, Nirula A, Gaynor R
Genomics 1992 Jul;13(3):665-71

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Characterization and chromosomal mapping of the gene encoding the cellular DNA binding protein ILF.

Li C, Lusis AJ, Sparkes R, Nirula A, Gaynor R
Genomics 1992 Jul;13(3):665-71

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ILF1 Antibody [NB100-1285] - (0.3ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: ILF1 Antibody [NB100-1285] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.