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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-20355 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p53AIP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is HL60 cell lysate.
- Concentration
- 1 mg/mL
Submitted references Germline mutations in apoptosis pathway genes in ovarian cancer; the functional role of a TP53I3 (PIG3) variant in ROS production and DNA repair.
Chaudhry SR, Lopes J, Levin NK, Kalpage H, Tainsky MA
Cell death discovery 2021 Mar 29;7(1):62
Cell death discovery 2021 Mar 29;7(1):62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HL60 cell lysate using a p53AIP1 polyclonal antibody (Product # PA5-20355) at (A) 4 and (B) 8 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of p53AIP1 in HL60 cell lysate with p53AIP1 Polyclonal Antibody (Product # PA5-20355) at (A) 4 and (B) 8 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HL60 cells using a p53AIP1 polyclonal antibody (Product # PA5-20355) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of p53AIP1 in HL60 cells with p53AIP1 Polyclonal Antibody (Product # PA5-20355) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of p53AIP1 in HL60 cells with p53AIP1 Polyclonal Antibody (Product # PA5-20355) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HL60 cells using a p53AIP1 polyclonal antibody (Product # PA5-20355) at a 10 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Effect of TP53I3-S252X on homologous recombination repair. HeLa-DR-GFP cells carry two inactive GFP alleles, the first allele contains the SCE1 endonuclease and the second allele is truncated. The introduction of pcBASce-1 to the cells causes a double-stranded break in the first GFP allele and the second GFP allele acts as a template for the HRR of the lesion. GFP expression is a quantifiable measurement of HRR using flow cytometry. A Effect of siRNA knockdown of BRCA1, TP53AIP1, TP53I3, or TP53I3 with wildtype (TP53I3-WT) or mutant plasmid (TP53I3-S252X). B Calculated two-way t test for each condition compared with scramble-positive control. With an n = 7, p values
