- PA5-27505 - Invitrogen Antibodies LDHB antibody

Submitted references LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain.

Park JS, Saeed K, Jo MH, Kim MW, Lee HJ, Park CB, Lee G, Kim MO
Antioxidants (Basel, Switzerland) 2022 Jan 28;11(2)

R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m(6)A/PFKP/LDHB axis.

Qing Y, Dong L, Gao L, Li C, Li Y, Han L, Prince E, Tan B, Deng X, Wetzel C, Shen C, Gao M, Chen Z, Li W, Zhang B, Braas D, Ten Hoeve J, Sanchez GJ, Chen H, Chan LN, Chen CW, Ann D, Jiang L, Müschen M, Marcucci G, Plas DR, Li Z, Su R, Chen J
Molecular cell 2021 Mar 4;81(5):922-939.e9

Quantitative Proteomic Approach Reveals Altered Metabolic Pathways in Response to the Inhibition of Lysine Deacetylases in A549 Cells under Normoxia and Hypoxia.

Martín-Bernabé A, Tarragó-Celada J, Cunin V, Michelland S, Cortés R, Poignant J, Boyault C, Rachidi W, Bourgoin-Voillard S, Cascante M, Seve M
International journal of molecular sciences 2021 Mar 25;22(7)

Branched-chain ketoacids secreted by glioblastoma cells via MCT1 modulate macrophage phenotype.

Silva LS, Poschet G, Nonnenmacher Y, Becker HM, Sapcariu S, Gaupel AC, Schlotter M, Wu Y, Kneisel N, Seiffert M, Hell R, Hiller K, Lichter P, Radlwimmer B
EMBO reports 2017 Dec;18(12):2172-2185

Lactate metabolism is associated with mammalian mitochondria.

Chen YJ, Mahieu NG, Huang X, Singh M, Crawford PA, Johnson SL, Gross RW, Schaefer J, Patti GJ
Nature chemical biology 2016 Nov;12(11):937-943

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of LDH-B using 30 µg of A) A549 (B) H1299 (C) HCT116 and D) MCF-7 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a LDH-B polyclonal antibody (Product # PA5-27505) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of LDH-B using 50 µg mouse brain extract. Samples were loaded onto a 10% SDS-PAGE gel and probed with a LDH-B polyclonal antibody (Product # PA5-27505) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of LDH-B using 50 µg rat brain extract. Samples were loaded onto a 10% SDS-PAGE gel and probed with a LDH-B polyclonal antibody (Product # PA5-27505) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of LDHB was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a LDHB Polyclonal Antibody (Product # PA5-27505) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using LDHB Polyclonal Antibody (Product # PA5-27505). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHB Polyclonal Antibody (Product # PA5-27505) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of LDHB was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a LDHB Polyclonal Antibody (Product # PA5-27505) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-LDHB Polyclonal Antibody (Product # PA5-27505) and a 35 kDa band corresponding to L-lactate dehydrogenase B chain was observed across MDA-MB-231, HeLa, Jurkat and Mouse Kidney. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), Hep G2 (Lane 4), MCF7 (Lane 5) and Mouse Kidney (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:8000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of LDH-B in methanol-fixed HeLa cells using a LDH-B polyclonal antibody (Product # PA5-27505) at a 1:50 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: LDHB Polyclonal Antibody detects LDH-B protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. LDH-B stained by LDHB Polyclonal Antibody (Product # PA5-27505) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: LDHB Polyclonal Antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. LDHB Polyclonal Antibody (Product # PA5-27505) dilution: 1:200. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Effect of KDAC inhibition on enzyme activities in A549 cells under normoxia and hypoxia. ( A , B ) The ATP- dependent 6-phosphofructokinase (PFK1) ( A ) and lactate dehydrogenase (LDH) ( B ) enzymatic activities were measured after 24 h of incubation, and activities were normalized to intracellular protein content in each condition. A549 cells were treated with 1 muM of TSA, 20 mM of NAM, and both 1 muM TSA and 20 mM NAM for 24 h of incubation under normoxia and hypoxia. Cells incubated in medium without KDACIs served as control. Bars represent the means +- standard error of the mean of three independent experiments. The asterisks above bars indicate statistically significant differences compared to normoxic control cells. The asterisks above curly brackets indicate statistically significant differences between hypoxic and normoxic treatments and between hypoxic treatments and hypoxic control cells. Statistical significance was assessed by a two-tailed Student''s t -test. *, p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Schematic diagram showing the generation of Ldhb -/- mouse, experimental design, and verification of Ldhb -/- model. ( A ) Schematic diagram illustrating the Cre-loxP and Flp-FRT system using pBS- Ldhb KO vectors. Ldhb -/- mice were generated through gene knockout at the exon 3 loci. ( B ) Schematic diagram of experimental design, showing the period of osmotin treatment in Ldhb -/- mice and behavior test. ( C , D ) The Western blot analysis showing the expression of LDHB in the cortex, hippocampus, kidney, and liver of Ldhb -/- and WT mice. beta-actin was used as a loading control. The bands were quantified using ImageJ software, and the differences are represented by histograms. ( E ) Confocal microscopic images showing the expression of LDHB in the cortex and hippocampus of Ldhb -/- , WT mice. ( F ) Immunohistochemistry staining for LDHB in the cortex and hippocampus of Ldhb -/- , WT mice.

PA5-27505

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